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Once administered the construct in the cell the nucleic acid encoding the construct of can be placed enhancement of bone cell spheroids and express themselves in different Places. These cells reach confluent densities, and then "stack" forming multi-layered cellular structures called bone nodules that mineralize their surrounding extracellular matrix. Specifically, the present invention relates to a process for producing ex vivo mammalian bone by obtaining an osteogenic or bone precursor cell; culturing the cell in serum-free conditions in the presence of one or more osteogenic growth factors; and establish cell cultures at cell densities that allow the formation of a bone-containing bone cell spheroid, whereby bone is formed within bone cell spheroid cells.
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